Gene Cloning Strategies

Part 1: Introduction

shot gun experiment explanation. Cosmid DNA. sonication. random fragments.

Part 2:

Applications, Human Genome project, Genome sequencing, whole Genome sequencing and genetic modification.

part 3:

Gene library

   Genomic library

     a) Nuclear Genomic library

     b) Organelle Genomic library

Construction of Genomic library

     Screening of genomic library

     Diagram explanation

   colony hybridization

   plaque hybridization

application of genomic library

storage of genomic library

part 4:

CDNA library

Diagram and definition

construction of CDNA library

   isolation mRNA

   CDNAs into vector

suitable host

screening

part 5:

CDNA library continues and applications

Disadvantages and Genomic library vs CDNA library.

strategies:

Cancer, including genitourinary malignancy, is a consequence of accumulated genetic aberrations in genes involved in crucial regulatory pathways. The result is a deregulation of cellular behavior, leading to neoplastic transformation, uncontrolled cell proliferation and acquisition of metastatic ability. The development and perfection of techniques in the field of recombinant DNA technology, gene cloning, (differential) analysis of gene expression, and sequencing of genes and proteins have provided a wealth of information about the genetic aberrations associated with cancer development. This “recombinant DNA and gene cloning” technology and the recently developed DNA chip technology may provide new molecular diagnostic tools. Furthermore, the technology of gene cloning in combination with the progress in in vivo gene delivery techniques offers new treatment modalities, like gene therapy, additional to conventional therapies. This review is intended to provide a general introduction to the fundamentals or strategies of recombinant DNA and gene cloning techniques as a basis for understanding the rapidly expanding range of new diagnostic and therapeutic opportunities. Some illustrative examples are provided, addressing basic and biomedical research and possible clinical applications in genitourinary oncology.